Part:BBa_K1362901:Design

C-terminal tripleSWAGtag (C-intein)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

During the design process it was a the utmost concern to achieve greatest SWAG. The sequence was therefore codon-optimised for E.Coli K12.

Source

The SWAG CDS was extracted from my genome where it occurs in high quantities and in an extremely repetitive pattern.